Postdoctoral Research Fellow in In situ Structural Biology

This role will be based on the university campus. We are open to discussing flexible working arrangements., The project builds on recent work from the laboratory on quality control mechanisms [1,2] and the mode of action of signalling proteins [3], which the successful applicant will extend into a native cellular context using in situ cryoET and correlative imaging.

Are you an ambitious researcher looking for your next challenge? Do you have an established background in in situ structural biology»? Do you want to further your career in one of the UKs leading research intensive Universities?, You should have (or be very close to completing) a PhD in in situ Structural Biology, or a related discipline. You should have a strong background in cryoEM, CryoET or related methods, and experience with setting up pipelines for cryoET data processing and/or FIB milling. Experience with in situ structural biology is essential.

We seek to appoint a Postdoctoral Research Fellow to the research team led by Prof. Elton Zeqiraj in the Faculty of Biological Sciences at the University of Leeds, Leeds, UK. The position is initially available for 3 years. The successful applicant will undertake in situ structural analysis using advanced cryoEM, cryoET and cutting-edge correlative microscopy methods. A major aspect of the post is setting up new pipelines for in situ structural biology and cryoET from cell systems using modern techniques to study ribosome quality control mechanisms. In addition, you will resolve the mode of action and target engagement of signalling proteins using signal particle cryoEM and sub-tomogram averaging. The position will suit someone interested in protein structure, in situ imaging / structural biology and cell signalling., The Astbury Centre for Structural & Molecular Biology at the University of Leeds has excellent facilities for in situ structural biology and is fully equipped for cryo-electron microscopy (two Titian Krios 300 kV with Falcon-4 detectors and Selectris energy filters), FIB-milling (Hydra Bio plasma-FIB) high pressure freezing equipment. Computational resources with dedicated workstations are available in the Zeqiraj laboratory and through high performance computer clusters within the University.
Additionally, the centre has protein crystallography and protein production facilities with expertise in multi-subunit expression in insect cell, yeast and bacterial expression systems. Other facilities provide capabilities for biophysical interactions, including the latest Biacore, MST, Dianthus (Nanotemper), ITC and Mass Photometry instruments. The University provides state-of-the-art infrastructure for insect cell and mammalian cell culture, high-throughput screening, imaging, mammalian genetics, chemical biology and proteomics.